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Hematopoietic Stem Cell Protocols 2e
Second Edition First Indian Reprint 2009 ,
326 pages, 43 ill, 4 in Colour Hard Cover,
Rs. 1295 /- For Sale in India, Pakistan, Nepal, Bangladesh & Sri lanka Only
About The Book
Table Of Contents
About The Author
"With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology™ series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Second Edition is a much needed technical resource in the critically important field of stem cell investigation. "
"Part I. Overview 1. The Expanding Tool Kit for Hematopoietic Stem Cell Research Part II. Stem Cell Enrichment and Analysis 2. Isolation of Quiescent Murine Hematopoietic Stem cells by Homing Properties 3. Mobilization of Hematopoietic Stem and Progenitor cells (HSPC) in Mice 4. Transmigration of Human CD34+ cells 5. Flow Cytometry Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells 6. Analysis of Apoptosis in HSC by Flow Cytometry Part III. In vitro Assays and Differentiation 7. In vitro Hematopoietic Differentiation of Murine Embryonic Stem (ES) Cells 8. Hematopoietic Development of Human Embryonic Stem Cells in Culture 9. In vitro Human T Cell Development Directed by Notch-ligand Interactions 10. In vitro Assays for Cobblestone Area Forming Cells, LTC-IC, and CFU-C Part IV. Transplantation Assays for Mouse and Human HSC 11. HSC Transplant in Mice by Intra Femoral Injection 12. Hematopoietic Stem Cell Transplant into Non-myeloablated W/Wv mice to Detect Steady-state Engraftment Defects 13. Isolation and Functional Characterization of Side Population Stem Cells 14. Transplantation of Chimeric Fetal Liver to Study Hematopoiesis 15. Immunodeficient Mouse Models to Study Human Stem Cell-mediated Tissue Repair Part V. Genetic Modification of HSCs and Imaging Engraftment 16. Retroviral Transduction of Murine Hematopoietic Stem Cells 17. Lentiviral Gene Transduction of Mouse and Human Stem Cells 18. Retroviral Integration Site Analysis in Hematopoietic Stem Cells 19. A Detailed Protocol for BAC Recombineering to Study Essential Genes in Stem Cells 20. Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models "
Kevin D. Bunting, Department of Medicine, Division of Hematology - Oncology, Center for Stem Cell and Regenerative Medicine, Case Western Reserve University, Cleveland,OH.
Students of Stem cells
Tool Kit for Hematopoietic Stem Cell Research, Protocols, Flow Cytometry Based Cell Cycle Measurement, Bunting
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