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Germline Stem Cells
First Indian Reprint 2009 ,
286 pages,, 57 ill, Hard Cover,
Rs. 995 /- For Sale in India, Pakistan, Nepal, Bangladesh & Sri lanka Only
About The Book
Table Of Contents
About The Author
"The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.Following the highly successful Methods in Molecular Biology™ Series format, each chapter includes a brief introduction to the subject,a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research. Features : 1. Readily reproducible protocols for the manipulation of germline stem cells in model organism. 2. State of the art methods optimized by leading experts in germline stem cells. 3. Identification and regulation of germline stem cells in model organisms 4. Current techniques used in invitro culture and application in germline stem cells 5. Presents sophisticated genetic, molecular, and cellular techniques used in germline stem cells research."
Preface Part I Identification and Regulation of Germline Stem Cells in Model Organisms 1. The Development of Germline Stem Cells in Drosophila 2.Analusis of the C.elegans Germline Stem Cell Region 3.Immunohistological Techniques for Studying the Drosophile Male Germline Stem Cell. 4. Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells. 5. Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect. 6. High Resolution Light Microscopic Characterization of Spermatogonia. 7. Identification and Characteization of Spermatogonial Subtypes and their expansion in Whole Mounts and Tissue Sections From Primate Testes. 8. Epigenetic Control in Male Germ Cells; A Transillumination Assisted Microdissection Method for the Analysis of Development Regulated Events 9. GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro. Part II InVitro Culture Applications of Germline Stem Cells 10. Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts. 11. Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice. 12. Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture. 13. Deriving Mouse Spermatogonial Stem Cell Lines 14. Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes. 15. Testicular Germ Cell Tumors in Mice: New ways to a Study a Genetically Complex Trait. 16. Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries. Index.
Steven X. Hou, National Cancer Institute, Frederick, MD. Shree Ram Singh, National Cancer Institute, Frederick, MD.
"This book provides background information and protocols for identifying, isolating, characterizing, and manipulating … germline stem cells. … This book is written for basic science and clinical researchers … as well as those interested in development and reproductive biology. Biomedical scientists and clinicians … will also appreciate the wealth of new information. … This is an outstanding contribution to the literature in germ cell biology. ... will be valued by all those interested in development of the germ lineage and stem cell biology." (Bruce A. Fenderson, Doody’s Review Service, October, 2008)
Students of Stem Cell
Drosophile Male Germline Stem Cell, Spermatogonia, Transillumination Assisted Microdissection, GDNF Maintains Mouse Spermatogonial Stem Cells, Testicular Germ Cell Tumors in Mice
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